Metagenomic methods for microbial ecologists
  • Day 1: Introduction
    • Preprocessing & quality filtering using a Snakemake workflow
    • Introduction to Snakemake
    • Launch Snakemake
    • Create a rule to unzip FASTQ files
    • Creating quality plots using FASTQC
    • Trim low quality reads and remove adapters
    • Create quality plots of the trimmed data
    • Create quality plots using R
    • Merge paired-end Illumina data
  • Day 4: Metagenomic assembly and binning
    • Installing perl-doc (to view some help files for ESOM)
    • Qality checking your data
    • running cutadapt
    • Running mira
    • Binning
    • ESOM
    • crAss
    • Differential coverage: manual
    • groopM
 
Metagenomic methods for microbial ecologists
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  • Welcome to Metagenomic methods for microbial ecologists’s documentation!
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Welcome to Metagenomic methods for microbial ecologists’s documentation!¶

Contents:

  • Day 1: Introduction
    • Preprocessing & quality filtering using a Snakemake workflow
    • Introduction to Snakemake
    • Launch Snakemake
    • Create a rule to unzip FASTQ files
    • Creating quality plots using FASTQC
    • Trim low quality reads and remove adapters
    • Create quality plots of the trimmed data
    • Create quality plots using R
    • Merge paired-end Illumina data
  • Day 4: Metagenomic assembly and binning
    • Installing perl-doc (to view some help files for ESOM)
    • Qality checking your data
    • running cutadapt
    • Running mira
    • Binning
    • ESOM
    • crAss
    • Differential coverage: manual
    • groopM
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© Copyright 2014, Mattias de Hollander, Bas Dutilh, Daan Speth.

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